Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_14_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-bs/v98/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 87834637 Number of alignments with a unique best hit from the different alignments: 25416779 Mapping efficiency: 28.9% Sequences with no alignments under any condition: 26593175 Sequences did not map uniquely: 35824683 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 5706998 ((converted) top strand) CT/GA: 5681196 ((converted) bottom strand) GA/CT: 7036812 (complementary to (converted) top strand) GA/GA: 6991773 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 157858861 Total methylated C's in CpG context: 24048953 Total methylated C's in CHG context: 699575 Total methylated C's in CHH context: 8140563 Total methylated C's in Unknown context: 7073 Total unmethylated C's in CpG context: 4179384 Total unmethylated C's in CHG context: 29973809 Total unmethylated C's in CHH context: 90816577 Total unmethylated C's in Unknown context: 50756 C methylated in CpG context: 85.2% C methylated in CHG context: 2.3% C methylated in CHH context: 8.2% C methylated in Unknown context (CN or CHN): 12.2% Bismark completed in 0d 1h 56m 27s