Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_14_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 87834637
Single-end alignments with a unique best hit 25416779
Sequences without alignments under any condition 26593175
Sequences that did not map uniquely 35824683
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 157858861
Methylated C's in CpG context 24048953
Methylated C's in CHG context 699575
Methylated C's in CHH context 8140563
Methylated C's in Unknown context 7073
Unmethylated C's in CpG context 4179384
Unmethylated C's in CHG context 29973809
Unmethylated C's in CHH context 90816577
Unmethylated C's in Unknown context 50756
Percentage methylation (CpG context) 85.2%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 8.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5706998 original top strand
CTOT 7036812 complementary to original top strand
CTOB 6991773 complementary to original bottom strand
OB 5681196 original bottom strand

Deduplication

Alignments analysed 25416779
Unique alignments 14328882
Duplicates removed 11087897
Duplicated alignments were found at 3309421 different positions

Cytosine Methylation after Extraction

Total C's analysed 87103963
Methylated C's in CpG context 11148831
Methylated C's in CHG context 439594
Methylated C's in CHH context 5924738
Unmethylated C's in CpG context 2386168
Unmethylated C's in CHG context 15349838
Unmethylated C's in CHH context 51854794
Percentage methylation (CpG context) 82.4%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 10.3%

M-Bias Plot