Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_13_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-bs/v98/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 64352550 Number of alignments with a unique best hit from the different alignments: 19626793 Mapping efficiency: 30.5% Sequences with no alignments under any condition: 18775282 Sequences did not map uniquely: 25950475 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 4395883 ((converted) top strand) CT/GA: 4355123 ((converted) bottom strand) GA/CT: 5461739 (complementary to (converted) top strand) GA/GA: 5414048 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 123096958 Total methylated C's in CpG context: 19042523 Total methylated C's in CHG context: 554503 Total methylated C's in CHH context: 6406969 Total methylated C's in Unknown context: 5721 Total unmethylated C's in CpG context: 3213173 Total unmethylated C's in CHG context: 24745143 Total unmethylated C's in CHH context: 69134647 Total unmethylated C's in Unknown context: 38192 C methylated in CpG context: 85.6% C methylated in CHG context: 2.2% C methylated in CHH context: 8.5% C methylated in Unknown context (CN or CHN): 13.0% Bismark completed in 0d 1h 25m 48s