Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_13_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 64352550
Single-end alignments with a unique best hit 19626793
Sequences without alignments under any condition 18775282
Sequences that did not map uniquely 25950475
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 123096958
Methylated C's in CpG context 19042523
Methylated C's in CHG context 554503
Methylated C's in CHH context 6406969
Methylated C's in Unknown context 5721
Unmethylated C's in CpG context 3213173
Unmethylated C's in CHG context 24745143
Unmethylated C's in CHH context 69134647
Unmethylated C's in Unknown context 38192
Percentage methylation (CpG context) 85.6%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 8.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4395883 original top strand
CTOT 5461739 complementary to original top strand
CTOB 5414048 complementary to original bottom strand
OB 4355123 original bottom strand

Deduplication

Alignments analysed 19626793
Unique alignments 11158024
Duplicates removed 8468769
Duplicated alignments were found at 2605304 different positions

Cytosine Methylation after Extraction

Total C's analysed 68398224
Methylated C's in CpG context 9042404
Methylated C's in CHG context 342660
Methylated C's in CHH context 4601823
Unmethylated C's in CpG context 1816241
Unmethylated C's in CHG context 12804713
Unmethylated C's in CHH context 39790383
Percentage methylation (CpG context) 83.3%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 10.4%

M-Bias Plot