Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_12_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 59459431
Single-end alignments with a unique best hit 20027644
Sequences without alignments under any condition 17427831
Sequences that did not map uniquely 22003956
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 119680665
Methylated C's in CpG context 13746910
Methylated C's in CHG context 459821
Methylated C's in CHH context 6669855
Methylated C's in Unknown context 4804
Unmethylated C's in CpG context 3405203
Unmethylated C's in CHG context 23855059
Unmethylated C's in CHH context 71543817
Unmethylated C's in Unknown context 33356
Percentage methylation (CpG context) 80.1%
Percentage methylation (CHG context) 1.9%
Percentage methylation (CHH context) 8.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4488497 original top strand
CTOT 5561933 complementary to original top strand
CTOB 5521382 complementary to original bottom strand
OB 4455831 original bottom strand

Deduplication

Alignments analysed 20027643
Unique alignments 13561841
Duplicates removed 6465802
Duplicated alignments were found at 2597927 different positions

Cytosine Methylation after Extraction

Total C's analysed 80149813
Methylated C's in CpG context 8313907
Methylated C's in CHG context 333592
Methylated C's in CHH context 5271016
Unmethylated C's in CpG context 2330165
Unmethylated C's in CHG context 15191561
Unmethylated C's in CHH context 48709572
Percentage methylation (CpG context) 78.1%
Percentage methylation (CHG context) 2.1%
Percentage methylation (CHH context) 9.8%

M-Bias Plot