Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_11_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 54458572
Single-end alignments with a unique best hit 15624995
Sequences without alignments under any condition 16588302
Sequences that did not map uniquely 22245275
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 100017389
Methylated C's in CpG context 17631307
Methylated C's in CHG context 489793
Methylated C's in CHH context 5187559
Methylated C's in Unknown context 4922
Unmethylated C's in CpG context 2899970
Unmethylated C's in CHG context 19768605
Unmethylated C's in CHH context 54040155
Unmethylated C's in Unknown context 34485
Percentage methylation (CpG context) 85.9%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 8.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3520857 original top strand
CTOT 4325599 complementary to original top strand
CTOB 4288436 complementary to original bottom strand
OB 3490103 original bottom strand

Deduplication

Alignments analysed 15624995
Unique alignments 8127256
Duplicates removed 7497739
Duplicated alignments were found at 1897754 different positions

Cytosine Methylation after Extraction

Total C's analysed 50434695
Methylated C's in CpG context 7064178
Methylated C's in CHG context 278667
Methylated C's in CHH context 3575067
Unmethylated C's in CpG context 1611371
Unmethylated C's in CHG context 9128258
Unmethylated C's in CHH context 28777154
Percentage methylation (CpG context) 81.4%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 11.1%

M-Bias Plot