Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_10_s456_trimmed.fq

Data processed at 00:07 on 2022-07-05


Alignment Stats

Sequences analysed in total 78221457
Single-end alignments with a unique best hit 25374001
Sequences without alignments under any condition 21899233
Sequences that did not map uniquely 30948223
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 159318003
Methylated C's in CpG context 24571414
Methylated C's in CHG context 738291
Methylated C's in CHH context 8532254
Methylated C's in Unknown context 7636
Unmethylated C's in CpG context 4429949
Unmethylated C's in CHG context 33811864
Unmethylated C's in CHH context 87234231
Unmethylated C's in Unknown context 52101
Percentage methylation (CpG context) 84.7%
Percentage methylation (CHG context) 2.1%
Percentage methylation (CHH context) 8.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6511929 original top strand
CTOT 6239306 complementary to original top strand
CTOB 6176648 complementary to original bottom strand
OB 6446118 original bottom strand

Deduplication

Alignments analysed 25374001
Unique alignments 8878355
Duplicates removed 16495646
Duplicated alignments were found at 3861835 different positions

Cytosine Methylation after Extraction

Total C's analysed 54346071
Methylated C's in CpG context 6587591
Methylated C's in CHG context 290156
Methylated C's in CHH context 4259112
Unmethylated C's in CpG context 1552509
Unmethylated C's in CHG context 10190620
Unmethylated C's in CHH context 31466083
Percentage methylation (CpG context) 80.9%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 11.9%

M-Bias Plot