Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/8_ACTTGA_L001_R1_001_trimmed.fq.gz (version: v0.22.3) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-bs/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 13949213 Number of alignments with a unique best hit from the different alignments: 6076830 Mapping efficiency: 43.6% Sequences with no alignments under any condition: 1810974 Sequences did not map uniquely: 6061409 Sequences which were discarded because genomic sequence could not be extracted: 5824 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3039408 ((converted) top strand) CT/GA: 3031598 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 32761745 Total methylated C's in CpG context: 1157034 Total methylated C's in CHG context: 73009 Total methylated C's in CHH context: 337279 Total methylated C's in Unknown context: 5050 Total unmethylated C's in CpG context: 3716117 Total unmethylated C's in CHG context: 6485444 Total unmethylated C's in CHH context: 20992862 Total unmethylated C's in Unknown context: 81686 C methylated in CpG context: 23.7% C methylated in CHG context: 1.1% C methylated in CHH context: 1.6% C methylated in Unknown context (CN or CHN): 5.8% Bismark completed in 0d 0h 19m 43s