Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/8_ACTTGA_L001_R1_001_trimmed.fq.gz

Data processed at 13:02:15 on 2021-07-02


Alignment Stats

Sequences analysed in total 13949213
Single-end alignments with a unique best hit 6076830
Sequences without alignments under any condition 1810974
Sequences that did not map uniquely 6061409
Genomic sequence context not extractable (edges of chromosomes) 5824

Cytosine Methylation

Total C's analysed 32761745
Methylated C's in CpG context 1157034
Methylated C's in CHG context 73009
Methylated C's in CHH context 337279
Methylated C's in Unknown context 5050
Unmethylated C's in CpG context 3716117
Unmethylated C's in CHG context 6485444
Unmethylated C's in CHH context 20992862
Unmethylated C's in Unknown context 81686
Percentage methylation (CpG context) 23.7%
Percentage methylation (CHG context) 1.1%
Percentage methylation (CHH context) 1.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3039408 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3031598 original bottom strand

Deduplication

Alignments analysed 6071006
Unique alignments 5214639
Duplicates removed 856367
Duplicated alignments were found at 501309 different positions

Cytosine Methylation after Extraction

Total C's analysed 28169285
Methylated C's in CpG context 966984
Methylated C's in CHG context 60729
Methylated C's in CHH context 288912
Unmethylated C's in CpG context 3106620
Unmethylated C's in CHG context 5461621
Unmethylated C's in CHH context 18284419
Percentage methylation (CpG context) 23.7%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot