Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/7_CAGATC_L001_R1_001_trimmed.fq.gz (version: v0.22.3) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-bs/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 10113854 Number of alignments with a unique best hit from the different alignments: 4409032 Mapping efficiency: 43.6% Sequences with no alignments under any condition: 1304913 Sequences did not map uniquely: 4399909 Sequences which were discarded because genomic sequence could not be extracted: 3991 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2205964 ((converted) top strand) CT/GA: 2199077 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 23601212 Total methylated C's in CpG context: 902662 Total methylated C's in CHG context: 39892 Total methylated C's in CHH context: 183434 Total methylated C's in Unknown context: 2936 Total unmethylated C's in CpG context: 2547688 Total unmethylated C's in CHG context: 4669084 Total unmethylated C's in CHH context: 15258452 Total unmethylated C's in Unknown context: 56865 C methylated in CpG context: 26.2% C methylated in CHG context: 0.8% C methylated in CHH context: 1.2% C methylated in Unknown context (CN or CHN): 4.9% Bismark completed in 0d 0h 14m 41s