Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/7_CAGATC_L001_R1_001_trimmed.fq.gz

Data processed at 13:02:15 on 2021-07-02


Alignment Stats

Sequences analysed in total 10113854
Single-end alignments with a unique best hit 4409032
Sequences without alignments under any condition 1304913
Sequences that did not map uniquely 4399909
Genomic sequence context not extractable (edges of chromosomes) 3991

Cytosine Methylation

Total C's analysed 23601212
Methylated C's in CpG context 902662
Methylated C's in CHG context 39892
Methylated C's in CHH context 183434
Methylated C's in Unknown context 2936
Unmethylated C's in CpG context 2547688
Unmethylated C's in CHG context 4669084
Unmethylated C's in CHH context 15258452
Unmethylated C's in Unknown context 56865
Percentage methylation (CpG context) 26.2%
Percentage methylation (CHG context) 0.8%
Percentage methylation (CHH context) 1.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2205964 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 2199077 original bottom strand

Deduplication

Alignments analysed 4405041
Unique alignments 3911895
Duplicates removed 493146
Duplicated alignments were found at 308035 different positions

Cytosine Methylation after Extraction

Total C's analysed 21041768
Methylated C's in CpG context 777012
Methylated C's in CHG context 34534
Methylated C's in CHH context 160956
Unmethylated C's in CpG context 2260229
Unmethylated C's in CHG context 4089010
Unmethylated C's in CHH context 13720027
Percentage methylation (CpG context) 25.6%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot