Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/6_GCCAAT_L001_R1_001_trimmed.fq.gz

Data processed at 13:02:15 on 2021-07-02


Alignment Stats

Sequences analysed in total 12175584
Single-end alignments with a unique best hit 5283036
Sequences without alignments under any condition 1645371
Sequences that did not map uniquely 5247177
Genomic sequence context not extractable (edges of chromosomes) 4855

Cytosine Methylation

Total C's analysed 28456278
Methylated C's in CpG context 1059882
Methylated C's in CHG context 82650
Methylated C's in CHH context 366373
Methylated C's in Unknown context 4344
Unmethylated C's in CpG context 3213576
Unmethylated C's in CHG context 5596516
Unmethylated C's in CHH context 18137281
Unmethylated C's in Unknown context 72858
Percentage methylation (CpG context) 24.8%
Percentage methylation (CHG context) 1.5%
Percentage methylation (CHH context) 2.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2637183 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 2640998 original bottom strand

Deduplication

Alignments analysed 5278181
Unique alignments 4559373
Duplicates removed 718808
Duplicated alignments were found at 396795 different positions

Cytosine Methylation after Extraction

Total C's analysed 24571154
Methylated C's in CpG context 889762
Methylated C's in CHG context 68974
Methylated C's in CHH context 316202
Unmethylated C's in CpG context 2662328
Unmethylated C's in CHG context 4740224
Unmethylated C's in CHH context 15893664
Percentage methylation (CpG context) 25.0%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot