Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/5_ACAGTG_L001_R1_001_trimmed.fq.gz

Data processed at 13:02:15 on 2021-07-02


Alignment Stats

Sequences analysed in total 12231319
Single-end alignments with a unique best hit 5362176
Sequences without alignments under any condition 1580596
Sequences that did not map uniquely 5288547
Genomic sequence context not extractable (edges of chromosomes) 5099

Cytosine Methylation

Total C's analysed 28880897
Methylated C's in CpG context 1107017
Methylated C's in CHG context 63558
Methylated C's in CHH context 270074
Methylated C's in Unknown context 3973
Unmethylated C's in CpG context 3245245
Unmethylated C's in CHG context 5740229
Unmethylated C's in CHH context 18454774
Unmethylated C's in Unknown context 73414
Percentage methylation (CpG context) 25.4%
Percentage methylation (CHG context) 1.1%
Percentage methylation (CHH context) 1.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2683369 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 2673708 original bottom strand

Deduplication

Alignments analysed 5357077
Unique alignments 4593972
Duplicates removed 763105
Duplicated alignments were found at 414884 different positions

Cytosine Methylation after Extraction

Total C's analysed 24788653
Methylated C's in CpG context 925261
Methylated C's in CHG context 52497
Methylated C's in CHH context 231354
Unmethylated C's in CpG context 2665822
Unmethylated C's in CHG context 4827653
Unmethylated C's in CHH context 16086066
Percentage methylation (CpG context) 25.8%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot