Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/4_TGACCA_L001_R1_001_trimmed.fq.gz

Data processed at 13:02:15 on 2021-07-02


Alignment Stats

Sequences analysed in total 11408856
Single-end alignments with a unique best hit 5032565
Sequences without alignments under any condition 1433341
Sequences that did not map uniquely 4942950
Genomic sequence context not extractable (edges of chromosomes) 4685

Cytosine Methylation

Total C's analysed 26935252
Methylated C's in CpG context 1072508
Methylated C's in CHG context 49594
Methylated C's in CHH context 229961
Methylated C's in Unknown context 3738
Unmethylated C's in CpG context 2908879
Unmethylated C's in CHG context 5301050
Unmethylated C's in CHH context 17373260
Unmethylated C's in Unknown context 66047
Percentage methylation (CpG context) 26.9%
Percentage methylation (CHG context) 0.9%
Percentage methylation (CHH context) 1.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2517228 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 2510652 original bottom strand

Deduplication

Alignments analysed 5027880
Unique alignments 4391580
Duplicates removed 636300
Duplicated alignments were found at 381714 different positions

Cytosine Methylation after Extraction

Total C's analysed 23572884
Methylated C's in CpG context 920224
Methylated C's in CHG context 41289
Methylated C's in CHH context 194717
Unmethylated C's in CpG context 2474902
Unmethylated C's in CHG context 4564383
Unmethylated C's in CHH context 15377369
Percentage methylation (CpG context) 27.1%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot