Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/3_TTAGGC_L001_R1_001_trimmed.fq.gz

Data processed at 13:02:15 on 2021-07-02


Alignment Stats

Sequences analysed in total 11555321
Single-end alignments with a unique best hit 5090692
Sequences without alignments under any condition 1486768
Sequences that did not map uniquely 4977861
Genomic sequence context not extractable (edges of chromosomes) 4594

Cytosine Methylation

Total C's analysed 27529174
Methylated C's in CpG context 1070205
Methylated C's in CHG context 62804
Methylated C's in CHH context 261548
Methylated C's in Unknown context 4043
Unmethylated C's in CpG context 3122098
Unmethylated C's in CHG context 5441541
Unmethylated C's in CHH context 17570978
Unmethylated C's in Unknown context 69965
Percentage methylation (CpG context) 25.5%
Percentage methylation (CHG context) 1.1%
Percentage methylation (CHH context) 1.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2552558 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 2533540 original bottom strand

Deduplication

Alignments analysed 5086098
Unique alignments 4357690
Duplicates removed 728408
Duplicated alignments were found at 371562 different positions

Cytosine Methylation after Extraction

Total C's analysed 23490464
Methylated C's in CpG context 898799
Methylated C's in CHG context 41098
Methylated C's in CHH context 185348
Unmethylated C's in CpG context 2496282
Unmethylated C's in CHG context 4576943
Unmethylated C's in CHH context 15291994
Percentage methylation (CpG context) 26.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot