Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/2_CGATGT_L001_R1_001_trimmed.fq.gz (version: v0.22.3) Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-bs/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 11654467 Number of alignments with a unique best hit from the different alignments: 5095582 Mapping efficiency: 43.7% Sequences with no alignments under any condition: 1488195 Sequences did not map uniquely: 5070690 Sequences which were discarded because genomic sequence could not be extracted: 4635 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2548767 ((converted) top strand) CT/GA: 2542180 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 27480056 Total methylated C's in CpG context: 1053319 Total methylated C's in CHG context: 99856 Total methylated C's in CHH context: 434623 Total methylated C's in Unknown context: 4424 Total unmethylated C's in CpG context: 3002656 Total unmethylated C's in CHG context: 5357714 Total unmethylated C's in CHH context: 17531888 Total unmethylated C's in Unknown context: 66894 C methylated in CpG context: 26.0% C methylated in CHG context: 1.8% C methylated in CHH context: 2.4% C methylated in Unknown context (CN or CHN): 6.2% Bismark completed in 0d 0h 17m 7s