Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/2_CGATGT_L001_R1_001_trimmed.fq.gz

Data processed at 13:02:15 on 2021-07-02


Alignment Stats

Sequences analysed in total 11654467
Single-end alignments with a unique best hit 5095582
Sequences without alignments under any condition 1488195
Sequences that did not map uniquely 5070690
Genomic sequence context not extractable (edges of chromosomes) 4635

Cytosine Methylation

Total C's analysed 27480056
Methylated C's in CpG context 1053319
Methylated C's in CHG context 99856
Methylated C's in CHH context 434623
Methylated C's in Unknown context 4424
Unmethylated C's in CpG context 3002656
Unmethylated C's in CHG context 5357714
Unmethylated C's in CHH context 17531888
Unmethylated C's in Unknown context 66894
Percentage methylation (CpG context) 26.0%
Percentage methylation (CHG context) 1.8%
Percentage methylation (CHH context) 2.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2548767 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 2542180 original bottom strand

Deduplication

Alignments analysed 5090947
Unique alignments 4405660
Duplicates removed 685287
Duplicated alignments were found at 403647 different positions

Cytosine Methylation after Extraction

Total C's analysed 23808752
Methylated C's in CpG context 890995
Methylated C's in CHG context 83304
Methylated C's in CHH context 372107
Unmethylated C's in CpG context 2529106
Unmethylated C's in CHG context 4559099
Unmethylated C's in CHH context 15374141
Percentage methylation (CpG context) 26.1%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot