Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/1_ATCACG_L001_R1_001_trimmed.fq.gz

Data processed at 13:02:14 on 2021-07-02


Alignment Stats

Sequences analysed in total 9246318
Single-end alignments with a unique best hit 4067513
Sequences without alignments under any condition 1179217
Sequences that did not map uniquely 3999588
Genomic sequence context not extractable (edges of chromosomes) 3816

Cytosine Methylation

Total C's analysed 21885255
Methylated C's in CpG context 866566
Methylated C's in CHG context 50314
Methylated C's in CHH context 214171
Methylated C's in Unknown context 3116
Unmethylated C's in CpG context 2383138
Unmethylated C's in CHG context 4326724
Unmethylated C's in CHH context 14044342
Unmethylated C's in Unknown context 53884
Percentage methylation (CpG context) 26.7%
Percentage methylation (CHG context) 1.1%
Percentage methylation (CHH context) 1.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2034763 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 2028934 original bottom strand

Deduplication

Alignments analysed 4063697
Unique alignments 3567365
Duplicates removed 496332
Duplicated alignments were found at 291435 different positions

Cytosine Methylation after Extraction

Total C's analysed 19257520
Methylated C's in CpG context 739459
Methylated C's in CHG context 36852
Methylated C's in CHH context 166608
Unmethylated C's in CpG context 2050571
Unmethylated C's in CHG context 3746639
Unmethylated C's in CHH context 12517391
Percentage methylation (CpG context) 26.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot