Bismark report for: /gscratch/srlab/sr320/data/cg-big/timeout/zrtg3644_5_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/timeout/zrtg3644_5_R2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/roslin_M/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 44083656 Number of paired-end alignments with a unique best hit: 26804023 Mapping efficiency: 60.8% Sequence pairs with no alignments under any condition: 10278106 Sequence pairs did not map uniquely: 7001527 Sequence pairs which were discarded because genomic sequence could not be extracted: 112 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 6645834 ((converted) top strand) GA/CT/CT: 6738674 (complementary to (converted) top strand) GA/CT/GA: 6756378 (complementary to (converted) bottom strand) CT/GA/GA: 6663025 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 952207866 Total methylated C's in CpG context: 12628932 Total methylated C's in CHG context: 797225 Total methylated C's in CHH context: 3315787 Total methylated C's in Unknown context: 33109 Total unmethylated C's in CpG context: 109297758 Total unmethylated C's in CHG context: 164227970 Total unmethylated C's in CHH context: 661940194 Total unmethylated C's in Unknown context: 2328229 C methylated in CpG context: 10.4% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.4% Bismark completed in 0d 4h 13m 51s