Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-206_S27_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-206_S27_L004_R2_001_val_2.fq.gz (version: v0.21.0_dev) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/geoduck/v074/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 43502 Mapping efficiency: 43.5% Sequence pairs with no alignments under any condition: 43133 Sequence pairs did not map uniquely: 13365 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 20106 ((converted) top strand) GA/CT/CT: 1437 (complementary to (converted) top strand) GA/CT/GA: 1424 (complementary to (converted) bottom strand) CT/GA/GA: 20535 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1251141 Total methylated C's in CpG context: 50142 Total methylated C's in CHG context: 7138 Total methylated C's in CHH context: 52714 Total methylated C's in Unknown context: 752 Total unmethylated C's in CpG context: 127409 Total unmethylated C's in CHG context: 259611 Total unmethylated C's in CHH context: 754127 Total unmethylated C's in Unknown context: 2750 C methylated in CpG context: 28.2% C methylated in CHG context: 2.7% C methylated in CHH context: 6.5% C methylated in unknown context (CN or CHN): 21.5% Bismark completed in 3d 12h 27m 56s