Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-205_S26_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-205_S26_L004_R2_001_val_2.fq.gz

Data processed at 18:21 on 2019-07-02

Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 39958
Pairs without alignments under any condition 46562
Pairs that did not map uniquely 13480
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 1097117
Methylated C's in CpG context 45280
Methylated C's in CHG context 6524
Methylated C's in CHH context 51112
Methylated C's in Unknown context 748
Unmethylated C's in CpG context 111878
Unmethylated C's in CHG context 221485
Unmethylated C's in CHH context 660838
Unmethylated C's in Unknown context 2527
Percentage methylation (CpG context) 28.8%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 7.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 18151 original top strand
CTOT 1588 complementary to original top strand
CTOB 1573 complementary to original bottom strand
OB 18646 original bottom strand

Cytosine Methylation after Extraction

Total C's analysed 726538
Methylated C's in CpG context 30348
Methylated C's in CHG context 4210
Methylated C's in CHH context 30809
Unmethylated C's in CpG context 73360
Unmethylated C's in CHG context 146243
Unmethylated C's in CHH context 441568
Percentage methylation (CpG context) 29.3%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 6.5%

M-Bias Plot