Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-193_S22_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-193_S22_L003_R2_001_val_2.fq.gz (version: v0.21.0_dev) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/geoduck/v074/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 41523 Mapping efficiency: 41.5% Sequence pairs with no alignments under any condition: 46402 Sequence pairs did not map uniquely: 12075 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 19328 ((converted) top strand) GA/CT/CT: 1178 (complementary to (converted) top strand) GA/CT/GA: 1217 (complementary to (converted) bottom strand) CT/GA/GA: 19800 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1184436 Total methylated C's in CpG context: 46818 Total methylated C's in CHG context: 6259 Total methylated C's in CHH context: 47129 Total methylated C's in Unknown context: 663 Total unmethylated C's in CpG context: 116014 Total unmethylated C's in CHG context: 239367 Total unmethylated C's in CHH context: 728849 Total unmethylated C's in Unknown context: 2718 C methylated in CpG context: 28.8% C methylated in CHG context: 2.5% C methylated in CHH context: 6.1% C methylated in unknown context (CN or CHN): 19.6% Bismark completed in 3d 1h 42m 48s