Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-184_S18_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-184_S18_L003_R2_001_val_2.fq.gz (version: v0.21.0_dev) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/geoduck/v074/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 42466 Mapping efficiency: 42.5% Sequence pairs with no alignments under any condition: 47104 Sequence pairs did not map uniquely: 10430 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 20028 ((converted) top strand) GA/CT/CT: 1077 (complementary to (converted) top strand) GA/CT/GA: 1094 (complementary to (converted) bottom strand) CT/GA/GA: 20267 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1283864 Total methylated C's in CpG context: 48464 Total methylated C's in CHG context: 6495 Total methylated C's in CHH context: 46806 Total methylated C's in Unknown context: 595 Total unmethylated C's in CpG context: 126521 Total unmethylated C's in CHG context: 264415 Total unmethylated C's in CHH context: 791163 Total unmethylated C's in Unknown context: 2996 C methylated in CpG context: 27.7% C methylated in CHG context: 2.4% C methylated in CHH context: 5.6% C methylated in unknown context (CN or CHN): 16.6% Bismark completed in 2d 17h 6m 34s