Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-159_S6_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-159_S6_L002_R2_001_val_2.fq.gz (version: v0.21.0_dev) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/geoduck/v074/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 43035 Mapping efficiency: 43.0% Sequence pairs with no alignments under any condition: 47180 Sequence pairs did not map uniquely: 9785 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 20126 ((converted) top strand) GA/CT/CT: 1200 (complementary to (converted) top strand) GA/CT/GA: 1148 (complementary to (converted) bottom strand) CT/GA/GA: 20561 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1328729 Total methylated C's in CpG context: 51781 Total methylated C's in CHG context: 7253 Total methylated C's in CHH context: 52915 Total methylated C's in Unknown context: 761 Total unmethylated C's in CpG context: 126344 Total unmethylated C's in CHG context: 268067 Total unmethylated C's in CHH context: 822369 Total unmethylated C's in Unknown context: 2993 C methylated in CpG context: 29.1% C methylated in CHG context: 2.6% C methylated in CHH context: 6.0% C methylated in unknown context (CN or CHN): 20.3% Bismark completed in 1d 15h 25m 45s