Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-120_S32_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-120_S32_L005_R2_001_val_2.fq.gz

Data processed at 18:21 on 2019-07-02

Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 46211
Pairs without alignments under any condition 44326
Pairs that did not map uniquely 9463
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 1451901
Methylated C's in CpG context 48860
Methylated C's in CHG context 7255
Methylated C's in CHH context 35386
Methylated C's in Unknown context 393
Unmethylated C's in CpG context 148228
Unmethylated C's in CHG context 288741
Unmethylated C's in CHH context 923431
Unmethylated C's in Unknown context 3104
Percentage methylation (CpG context) 24.8%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 3.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 22193 original top strand
CTOT 511 complementary to original top strand
CTOB 540 complementary to original bottom strand
OB 22967 original bottom strand

Cytosine Methylation after Extraction

Total C's analysed 1074595
Methylated C's in CpG context 36923
Methylated C's in CHG context 5442
Methylated C's in CHH context 24216
Unmethylated C's in CpG context 109409
Unmethylated C's in CHG context 212041
Unmethylated C's in CHH context 686564
Percentage methylation (CpG context) 25.2%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 3.4%

M-Bias Plot