Bismark report for: /gscratch/scrubbed/sr320/0620/zr1394_all_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-genomes/v081/ with the specified options: -q --score-min L,0,-0.2 -p 14 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 1635584275 Number of alignments with a unique best hit from the different alignments: 507562199 Mapping efficiency: 31.0% Sequences with no alignments under any condition: 477689355 Sequences did not map uniquely: 650332721 Sequences which were discarded because genomic sequence could not be extracted: 496024 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 115629955 ((converted) top strand) CT/GA: 114795254 ((converted) bottom strand) GA/CT: 138900291 (complementary to (converted) top strand) GA/GA: 137740675 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3127742922 Total methylated C's in CpG context: 450929646 Total methylated C's in CHG context: 14053551 Total methylated C's in CHH context: 169276746 Total methylated C's in Unknown context: 84062 Total unmethylated C's in CpG context: 83521836 Total unmethylated C's in CHG context: 623336476 Total unmethylated C's in CHH context: 1786624667 Total unmethylated C's in Unknown context: 569646 C methylated in CpG context: 84.4% C methylated in CHG context: 2.2% C methylated in CHH context: 8.7% C methylated in Unknown context (CN or CHN): 12.9% Bismark completed in 4d 19h 52m 3s