Bismark

Bismark Processing Report

/gscratch/scrubbed/sr320/0620/zr1394_all_s456_trimmed.fq

Data processed at 04:12 on 2019-06-30


Alignment Stats

Sequences analysed in total 1635584275
Single-end alignments with a unique best hit 507562199
Sequences without alignments under any condition 477689355
Sequences that did not map uniquely 650332721
Genomic sequence context not extractable (edges of chromosomes) 496024

Cytosine Methylation

Total C's analysed 3127742922
Methylated C's in CpG context 450929646
Methylated C's in CHG context 14053551
Methylated C's in CHH context 169276746
Methylated C's in Unknown context 84062
Unmethylated C's in CpG context 83521836
Unmethylated C's in CHG context 623336476
Unmethylated C's in CHH context 1786624667
Unmethylated C's in Unknown context 569646
Percentage methylation (CpG context) 84.4%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 8.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 115629955 original top strand
CTOT 138900291 complementary to original top strand
CTOB 137740675 complementary to original bottom strand
OB 114795254 original bottom strand

Deduplication

Alignments analysed 507066175
Unique alignments 100277394
Duplicates removed 406788781
Duplicated alignments were found at 67040665 different positions

Cytosine Methylation after Extraction

Total C's analysed 589517746
Methylated C's in CpG context 54161439
Methylated C's in CHG context 3410986
Methylated C's in CHH context 51877126
Unmethylated C's in CpG context 18632781
Unmethylated C's in CHG context 98241295
Unmethylated C's in CHH context 363194119
Percentage methylation (CpG context) 74.4%
Percentage methylation (CHG context) 3.4%
Percentage methylation (CHH context) 12.5%

M-Bias Plot