Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_all_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 605809374
Single-end alignments with a unique best hit 146216889
Sequences without alignments under any condition 246381185
Sequences that did not map uniquely 213211300
Genomic sequence context not extractable (edges of chromosomes) 61826

Cytosine Methylation

Total C's analysed 912885367
Methylated C's in CpG context 132313268
Methylated C's in CHG context 4673170
Methylated C's in CHH context 63365512
Methylated C's in Unknown context 20620
Unmethylated C's in CpG context 25469052
Unmethylated C's in CHG context 171379800
Unmethylated C's in CHH context 515684565
Unmethylated C's in Unknown context 113058
Percentage methylation (CpG context) 83.9%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 10.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 33641201 original top strand
CTOT 39779165 complementary to original top strand
CTOB 39289786 complementary to original bottom strand
OB 33444911 original bottom strand

Deduplication

Alignments analysed 146155063
Unique alignments 46373119
Duplicates removed 99781944
Duplicated alignments were found at 16205715 different positions

Cytosine Methylation after Extraction

Total C's analysed 278742554
Methylated C's in CpG context 26906729
Methylated C's in CHG context 1781155
Methylated C's in CHH context 28939234
Unmethylated C's in CpG context 8635735
Unmethylated C's in CHG context 43946205
Unmethylated C's in CHH context 168533496
Percentage methylation (CpG context) 75.7%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 14.7%

M-Bias Plot