Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_9_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 77350922
Single-end alignments with a unique best hit 19984598
Sequences without alignments under any condition 30671788
Sequences that did not map uniquely 26694536
Genomic sequence context not extractable (edges of chromosomes) 7540

Cytosine Methylation

Total C's analysed 119671174
Methylated C's in CpG context 12630031
Methylated C's in CHG context 518512
Methylated C's in CHH context 8542027
Methylated C's in Unknown context 1880
Unmethylated C's in CpG context 3349469
Unmethylated C's in CHG context 21459404
Unmethylated C's in CHH context 73171731
Unmethylated C's in Unknown context 13241
Percentage methylation (CpG context) 79.0%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 10.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4526893 original top strand
CTOT 5500698 complementary to original top strand
CTOB 5442627 complementary to original bottom strand
OB 4506840 original bottom strand

Deduplication

Alignments analysed 19977058
Unique alignments 12812127
Duplicates removed 7164931
Duplicated alignments were found at 2565720 different positions

Cytosine Methylation after Extraction

Total C's analysed 75709462
Methylated C's in CpG context 6976233
Methylated C's in CHG context 355964
Methylated C's in CHH context 6308433
Unmethylated C's in CpG context 2143064
Unmethylated C's in CHG context 12824703
Unmethylated C's in CHH context 47101065
Percentage methylation (CpG context) 76.5%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 11.8%

M-Bias Plot