Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_8_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 38697189
Single-end alignments with a unique best hit 9523100
Sequences without alignments under any condition 15822075
Sequences that did not map uniquely 13352014
Genomic sequence context not extractable (edges of chromosomes) 3569

Cytosine Methylation

Total C's analysed 58792018
Methylated C's in CpG context 7971301
Methylated C's in CHG context 300645
Methylated C's in CHH context 4199146
Methylated C's in Unknown context 1156
Unmethylated C's in CpG context 1357932
Unmethylated C's in CHG context 10977916
Unmethylated C's in CHH context 33985078
Unmethylated C's in Unknown context 6970
Percentage methylation (CpG context) 85.4%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 11.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2127682 original top strand
CTOT 2651382 complementary to original top strand
CTOB 2622873 complementary to original bottom strand
OB 2117594 original bottom strand

Deduplication

Alignments analysed 9519531
Unique alignments 6568453
Duplicates removed 2951078
Duplicated alignments were found at 1150254 different positions

Cytosine Methylation after Extraction

Total C's analysed 39971460
Methylated C's in CpG context 4836003
Methylated C's in CHG context 216974
Methylated C's in CHH context 3271126
Unmethylated C's in CpG context 915478
Unmethylated C's in CHG context 7094192
Unmethylated C's in CHH context 23637687
Percentage methylation (CpG context) 84.1%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 12.2%

M-Bias Plot