Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_7_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 49730113
Single-end alignments with a unique best hit 12175088
Sequences without alignments under any condition 20602272
Sequences that did not map uniquely 16952753
Genomic sequence context not extractable (edges of chromosomes) 4774

Cytosine Methylation

Total C's analysed 74916181
Methylated C's in CpG context 9646533
Methylated C's in CHG context 391823
Methylated C's in CHH context 5377197
Methylated C's in Unknown context 1404
Unmethylated C's in CpG context 2085465
Unmethylated C's in CHG context 13912725
Unmethylated C's in CHH context 43502438
Unmethylated C's in Unknown context 8321
Percentage methylation (CpG context) 82.2%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 11.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2796387 original top strand
CTOT 3315619 complementary to original top strand
CTOB 3276555 complementary to original bottom strand
OB 2781753 original bottom strand

Deduplication

Alignments analysed 12170314
Unique alignments 8282510
Duplicates removed 3887804
Duplicated alignments were found at 1495485 different positions

Cytosine Methylation after Extraction

Total C's analysed 50230657
Methylated C's in CpG context 5722013
Methylated C's in CHG context 278091
Methylated C's in CHH context 4157227
Unmethylated C's in CpG context 1407766
Unmethylated C's in CHG context 8854701
Unmethylated C's in CHH context 29810859
Percentage methylation (CpG context) 80.3%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 12.2%

M-Bias Plot