Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_6_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 46455148
Single-end alignments with a unique best hit 12263620
Sequences without alignments under any condition 19313057
Sequences that did not map uniquely 14878471
Genomic sequence context not extractable (edges of chromosomes) 4333

Cytosine Methylation

Total C's analysed 72321697
Methylated C's in CpG context 6785652
Methylated C's in CHG context 357854
Methylated C's in CHH context 5655325
Methylated C's in Unknown context 974
Unmethylated C's in CpG context 1919729
Unmethylated C's in CHG context 13022398
Unmethylated C's in CHH context 44580739
Unmethylated C's in Unknown context 7575
Percentage methylation (CpG context) 77.9%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 11.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2761017 original top strand
CTOT 3390420 complementary to original top strand
CTOB 3357895 complementary to original bottom strand
OB 2749955 original bottom strand

Deduplication

Alignments analysed 12259287
Unique alignments 9168099
Duplicates removed 3091188
Duplicated alignments were found at 1424553 different positions

Cytosine Methylation after Extraction

Total C's analysed 53570122
Methylated C's in CpG context 4597895
Methylated C's in CHG context 267098
Methylated C's in CHH context 4504381
Unmethylated C's in CpG context 1438902
Unmethylated C's in CHG context 9316512
Unmethylated C's in CHH context 33445334
Percentage methylation (CpG context) 76.2%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 11.9%

M-Bias Plot