Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_5_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 47401736
Single-end alignments with a unique best hit 12477785
Sequences without alignments under any condition 19516870
Sequences that did not map uniquely 15407081
Genomic sequence context not extractable (edges of chromosomes) 4488

Cytosine Methylation

Total C's analysed 74333364
Methylated C's in CpG context 7469156
Methylated C's in CHG context 359359
Methylated C's in CHH context 5921080
Methylated C's in Unknown context 1192
Unmethylated C's in CpG context 2522221
Unmethylated C's in CHG context 13779482
Unmethylated C's in CHH context 44282066
Unmethylated C's in Unknown context 7439
Percentage methylation (CpG context) 74.8%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 11.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2851638 original top strand
CTOT 3409626 complementary to original top strand
CTOB 3370322 complementary to original bottom strand
OB 2841711 original bottom strand

Deduplication

Alignments analysed 12473297
Unique alignments 9178110
Duplicates removed 3295187
Duplicated alignments were found at 1470802 different positions

Cytosine Methylation after Extraction

Total C's analysed 54183369
Methylated C's in CpG context 4893430
Methylated C's in CHG context 270511
Methylated C's in CHH context 4694436
Unmethylated C's in CpG context 1878697
Unmethylated C's in CHG context 9626073
Unmethylated C's in CHH context 32820222
Percentage methylation (CpG context) 72.3%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 12.5%

M-Bias Plot