Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_4_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 44905078
Single-end alignments with a unique best hit 10995781
Sequences without alignments under any condition 18625519
Sequences that did not map uniquely 15283778
Genomic sequence context not extractable (edges of chromosomes) 4353

Cytosine Methylation

Total C's analysed 67752173
Methylated C's in CpG context 8917744
Methylated C's in CHG context 360092
Methylated C's in CHH context 4933503
Methylated C's in Unknown context 1221
Unmethylated C's in CpG context 1626331
Unmethylated C's in CHG context 12609014
Unmethylated C's in CHH context 39305489
Unmethylated C's in Unknown context 7467
Percentage methylation (CpG context) 84.6%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 11.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2474330 original top strand
CTOT 3046844 complementary to original top strand
CTOB 3015697 complementary to original bottom strand
OB 2454557 original bottom strand

Deduplication

Alignments analysed 10991428
Unique alignments 7374338
Duplicates removed 3617090
Duplicated alignments were found at 1354206 different positions

Cytosine Methylation after Extraction

Total C's analysed 44754848
Methylated C's in CpG context 5210066
Methylated C's in CHG context 253236
Methylated C's in CHH context 3757790
Unmethylated C's in CpG context 1067149
Unmethylated C's in CHG context 7898014
Unmethylated C's in CHH context 26568593
Percentage methylation (CpG context) 83.0%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 12.4%

M-Bias Plot