Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_3_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 48376729
Single-end alignments with a unique best hit 11710900
Sequences without alignments under any condition 20186374
Sequences that did not map uniquely 16479455
Genomic sequence context not extractable (edges of chromosomes) 4911

Cytosine Methylation

Total C's analysed 71201719
Methylated C's in CpG context 8686245
Methylated C's in CHG context 362509
Methylated C's in CHH context 5275881
Methylated C's in Unknown context 1358
Unmethylated C's in CpG context 1984216
Unmethylated C's in CHG context 12789657
Unmethylated C's in CHH context 42103211
Unmethylated C's in Unknown context 7803
Percentage methylation (CpG context) 81.4%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 11.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2643508 original top strand
CTOT 3235499 complementary to original top strand
CTOB 3198110 complementary to original bottom strand
OB 2628872 original bottom strand

Deduplication

Alignments analysed 11705989
Unique alignments 8087518
Duplicates removed 3618471
Duplicated alignments were found at 1381700 different positions

Cytosine Methylation after Extraction

Total C's analysed 48505780
Methylated C's in CpG context 5175437
Methylated C's in CHG context 259268
Methylated C's in CHH context 4088283
Unmethylated C's in CpG context 1368035
Unmethylated C's in CHG context 8268346
Unmethylated C's in CHH context 29346411
Percentage methylation (CpG context) 79.1%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 12.2%

M-Bias Plot