Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_2_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 46105140
Single-end alignments with a unique best hit 11724450
Sequences without alignments under any condition 19221903
Sequences that did not map uniquely 15158787
Genomic sequence context not extractable (edges of chromosomes) 4558

Cytosine Methylation

Total C's analysed 71191354
Methylated C's in CpG context 8594713
Methylated C's in CHG context 366860
Methylated C's in CHH context 5407980
Methylated C's in Unknown context 1227
Unmethylated C's in CpG context 1924039
Unmethylated C's in CHG context 13342035
Unmethylated C's in CHH context 41555727
Unmethylated C's in Unknown context 7283
Percentage methylation (CpG context) 81.7%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 11.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2639464 original top strand
CTOT 3242721 complementary to original top strand
CTOB 3212565 complementary to original bottom strand
OB 2625142 original bottom strand

Deduplication

Alignments analysed 11719892
Unique alignments 7995609
Duplicates removed 3724283
Duplicated alignments were found at 1559750 different positions

Cytosine Methylation after Extraction

Total C's analysed 48052045
Methylated C's in CpG context 5203176
Methylated C's in CHG context 260623
Methylated C's in CHH context 4107377
Unmethylated C's in CpG context 1320948
Unmethylated C's in CHG context 8570542
Unmethylated C's in CHH context 28589379
Percentage methylation (CpG context) 79.8%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 12.6%

M-Bias Plot