Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_1_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 46792286
Single-end alignments with a unique best hit 11528343
Sequences without alignments under any condition 19262205
Sequences that did not map uniquely 16001738
Genomic sequence context not extractable (edges of chromosomes) 4762

Cytosine Methylation

Total C's analysed 70539756
Methylated C's in CpG context 8878857
Methylated C's in CHG context 371279
Methylated C's in CHH context 5170660
Methylated C's in Unknown context 1202
Unmethylated C's in CpG context 2053585
Unmethylated C's in CHG context 13005205
Unmethylated C's in CHH context 41060170
Unmethylated C's in Unknown context 8096
Percentage methylation (CpG context) 81.2%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 11.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2628200 original top strand
CTOT 3158526 complementary to original top strand
CTOB 3126522 complementary to original bottom strand
OB 2610333 original bottom strand

Deduplication

Alignments analysed 11523581
Unique alignments 7910826
Duplicates removed 3612755
Duplicated alignments were found at 1394237 different positions

Cytosine Methylation after Extraction

Total C's analysed 47710874
Methylated C's in CpG context 5279763
Methylated C's in CHG context 263115
Methylated C's in CHH context 3985882
Unmethylated C's in CpG context 1425331
Unmethylated C's in CHG context 8348272
Unmethylated C's in CHH context 28408511
Percentage methylation (CpG context) 78.7%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 12.3%

M-Bias Plot