Alignment Stats
| Sequences analysed in total |
46792286 |
| Single-end alignments with a unique best hit |
11528343 |
| Sequences without alignments under any condition |
19262205 |
| Sequences that did not map uniquely |
16001738 |
| Genomic sequence context not extractable (edges of chromosomes) |
4762 |
|
|
Cytosine Methylation
| Total C's analysed |
70539756 |
| Methylated C's in CpG context |
8878857 |
| Methylated C's in CHG context |
371279 |
| Methylated C's in CHH context |
5170660 |
| Methylated C's in Unknown context |
1202 |
| Unmethylated C's in CpG context |
2053585 |
| Unmethylated C's in CHG context |
13005205 |
| Unmethylated C's in CHH context |
41060170 |
| Unmethylated C's in Unknown context |
8096 |
| Percentage methylation (CpG context) |
81.2% |
| Percentage methylation (CHG context) |
2.8% |
| Percentage methylation (CHH context) |
11.2% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
2628200 |
original top strand |
| CTOT |
3158526 |
complementary to original top strand |
| CTOB |
3126522 |
complementary to original bottom strand |
| OB |
2610333 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
11523581 |
| Unique alignments |
7910826 |
| Duplicates removed |
3612755 |
| Duplicated alignments were found at 1394237 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
47710874 |
| Methylated C's in CpG context |
5279763 |
| Methylated C's in CHG context |
263115 |
| Methylated C's in CHH context |
3985882 |
| Unmethylated C's in CpG context |
1425331 |
| Unmethylated C's in CHG context |
8348272 |
| Unmethylated C's in CHH context |
28408511 |
| Percentage methylation (CpG context) |
78.7% |
| Percentage methylation (CHG context) |
3.1% |
| Percentage methylation (CHH context) |
12.3% |
|
|
M-Bias Plot