Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_18_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 55048106
Single-end alignments with a unique best hit 14252437
Sequences without alignments under any condition 21991657
Sequences that did not map uniquely 18804012
Genomic sequence context not extractable (edges of chromosomes) 5287

Cytosine Methylation

Total C's analysed 86615880
Methylated C's in CpG context 10502357
Methylated C's in CHG context 403533
Methylated C's in CHH context 6221917
Methylated C's in Unknown context 1408
Unmethylated C's in CpG context 2318634
Unmethylated C's in CHG context 16473257
Unmethylated C's in CHH context 50696182
Unmethylated C's in Unknown context 10049
Percentage methylation (CpG context) 81.9%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 10.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3154293 original top strand
CTOT 3998948 complementary to original top strand
CTOB 3956463 complementary to original bottom strand
OB 3137446 original bottom strand

Deduplication

Alignments analysed 14247150
Unique alignments 9291908
Duplicates removed 4955242
Duplicated alignments were found at 1909975 different positions

Cytosine Methylation after Extraction

Total C's analysed 55767083
Methylated C's in CpG context 5940617
Methylated C's in CHG context 277785
Methylated C's in CHH context 4617003
Unmethylated C's in CpG context 1497305
Unmethylated C's in CHG context 10045285
Unmethylated C's in CHH context 33389088
Percentage methylation (CpG context) 79.9%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 12.1%

M-Bias Plot