Alignment Stats
| Sequences analysed in total |
42070546 |
| Single-end alignments with a unique best hit |
10118617 |
| Sequences without alignments under any condition |
17079542 |
| Sequences that did not map uniquely |
14872387 |
| Genomic sequence context not extractable (edges of chromosomes) |
4467 |
|
|
Cytosine Methylation
| Total C's analysed |
63322939 |
| Methylated C's in CpG context |
9682915 |
| Methylated C's in CHG context |
345329 |
| Methylated C's in CHH context |
4634028 |
| Methylated C's in Unknown context |
1427 |
| Unmethylated C's in CpG context |
1668241 |
| Unmethylated C's in CHG context |
12337321 |
| Unmethylated C's in CHH context |
34655105 |
| Unmethylated C's in Unknown context |
7659 |
| Percentage methylation (CpG context) |
85.3% |
| Percentage methylation (CHG context) |
2.7% |
| Percentage methylation (CHH context) |
11.8% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
2309784 |
original top strand |
| CTOT |
2770394 |
complementary to original top strand |
| CTOB |
2731360 |
complementary to original bottom strand |
| OB |
2302612 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
10114150 |
| Unique alignments |
6018753 |
| Duplicates removed |
4095397 |
| Duplicated alignments were found at 1338945 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
37059948 |
| Methylated C's in CpG context |
4814025 |
| Methylated C's in CHG context |
220677 |
| Methylated C's in CHH context |
3306570 |
| Unmethylated C's in CpG context |
949135 |
| Unmethylated C's in CHG context |
6739506 |
| Unmethylated C's in CHH context |
21030035 |
| Percentage methylation (CpG context) |
83.5% |
| Percentage methylation (CHG context) |
3.2% |
| Percentage methylation (CHH context) |
13.6% |
|
|
M-Bias Plot