Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_17_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 42070546
Single-end alignments with a unique best hit 10118617
Sequences without alignments under any condition 17079542
Sequences that did not map uniquely 14872387
Genomic sequence context not extractable (edges of chromosomes) 4467

Cytosine Methylation

Total C's analysed 63322939
Methylated C's in CpG context 9682915
Methylated C's in CHG context 345329
Methylated C's in CHH context 4634028
Methylated C's in Unknown context 1427
Unmethylated C's in CpG context 1668241
Unmethylated C's in CHG context 12337321
Unmethylated C's in CHH context 34655105
Unmethylated C's in Unknown context 7659
Percentage methylation (CpG context) 85.3%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 11.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2309784 original top strand
CTOT 2770394 complementary to original top strand
CTOB 2731360 complementary to original bottom strand
OB 2302612 original bottom strand

Deduplication

Alignments analysed 10114150
Unique alignments 6018753
Duplicates removed 4095397
Duplicated alignments were found at 1338945 different positions

Cytosine Methylation after Extraction

Total C's analysed 37059948
Methylated C's in CpG context 4814025
Methylated C's in CHG context 220677
Methylated C's in CHH context 3306570
Unmethylated C's in CpG context 949135
Unmethylated C's in CHG context 6739506
Unmethylated C's in CHH context 21030035
Percentage methylation (CpG context) 83.5%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 13.6%

M-Bias Plot