Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_16_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 74531056
Single-end alignments with a unique best hit 16887122
Sequences without alignments under any condition 30685115
Sequences that did not map uniquely 26958819
Genomic sequence context not extractable (edges of chromosomes) 7822

Cytosine Methylation

Total C's analysed 106385975
Methylated C's in CpG context 16804738
Methylated C's in CHG context 565878
Methylated C's in CHH context 7190353
Methylated C's in Unknown context 2962
Unmethylated C's in CpG context 3074886
Unmethylated C's in CHG context 19338243
Unmethylated C's in CHH context 59411877
Unmethylated C's in Unknown context 13297
Percentage methylation (CpG context) 84.5%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 10.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3891231 original top strand
CTOT 4594843 complementary to original top strand
CTOB 4530426 complementary to original bottom strand
OB 3862800 original bottom strand

Deduplication

Alignments analysed 16879300
Unique alignments 9152179
Duplicates removed 7727121
Duplicated alignments were found at 2138322 different positions

Cytosine Methylation after Extraction

Total C's analysed 56409754
Methylated C's in CpG context 7253055
Methylated C's in CHG context 334133
Methylated C's in CHH context 4911041
Unmethylated C's in CpG context 1659423
Unmethylated C's in CHG context 9414630
Unmethylated C's in CHH context 32837472
Percentage methylation (CpG context) 81.4%
Percentage methylation (CHG context) 3.4%
Percentage methylation (CHH context) 13.0%

M-Bias Plot