Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_15_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 67984205
Single-end alignments with a unique best hit 15694384
Sequences without alignments under any condition 28009193
Sequences that did not map uniquely 24280628
Genomic sequence context not extractable (edges of chromosomes) 6700

Cytosine Methylation

Total C's analysed 99648422
Methylated C's in CpG context 16146845
Methylated C's in CHG context 537672
Methylated C's in CHH context 6779395
Methylated C's in Unknown context 2641
Unmethylated C's in CpG context 2533863
Unmethylated C's in CHG context 18697431
Unmethylated C's in CHH context 54953216
Unmethylated C's in Unknown context 12429
Percentage methylation (CpG context) 86.4%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 11.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3556064 original top strand
CTOT 4328479 complementary to original top strand
CTOB 4260613 complementary to original bottom strand
OB 3542528 original bottom strand

Deduplication

Alignments analysed 15687684
Unique alignments 8276307
Duplicates removed 7411377
Duplicated alignments were found at 2004615 different positions

Cytosine Methylation after Extraction

Total C's analysed 51329402
Methylated C's in CpG context 6815335
Methylated C's in CHG context 313906
Methylated C's in CHH context 4527620
Unmethylated C's in CpG context 1340799
Unmethylated C's in CHG context 8807650
Unmethylated C's in CHH context 29524092
Percentage methylation (CpG context) 83.6%
Percentage methylation (CHG context) 3.4%
Percentage methylation (CHH context) 13.3%

M-Bias Plot