Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_14_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 87834637
Single-end alignments with a unique best hit 20190001
Sequences without alignments under any condition 35695594
Sequences that did not map uniquely 31949042
Genomic sequence context not extractable (edges of chromosomes) 8673

Cytosine Methylation

Total C's analysed 125655879
Methylated C's in CpG context 18386710
Methylated C's in CHG context 628262
Methylated C's in CHH context 8260723
Methylated C's in Unknown context 2658
Unmethylated C's in CpG context 3490698
Unmethylated C's in CHG context 22406312
Unmethylated C's in CHH context 72483174
Unmethylated C's in Unknown context 17691
Percentage methylation (CpG context) 84.0%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 10.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4568710 original top strand
CTOT 5564558 complementary to original top strand
CTOB 5504500 complementary to original bottom strand
OB 4543560 original bottom strand

Deduplication

Alignments analysed 20181328
Unique alignments 11066467
Duplicates removed 9114861
Duplicated alignments were found at 2605750 different positions

Cytosine Methylation after Extraction

Total C's analysed 67599507
Methylated C's in CpG context 8137184
Methylated C's in CHG context 381105
Methylated C's in CHH context 5714033
Unmethylated C's in CpG context 1906366
Unmethylated C's in CHG context 11097562
Unmethylated C's in CHH context 40363257
Percentage methylation (CpG context) 81.0%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 12.4%

M-Bias Plot