Alignment Stats
| Sequences analysed in total |
87834637 |
| Single-end alignments with a unique best hit |
20190001 |
| Sequences without alignments under any condition |
35695594 |
| Sequences that did not map uniquely |
31949042 |
| Genomic sequence context not extractable (edges of chromosomes) |
8673 |
|
|
Cytosine Methylation
| Total C's analysed |
125655879 |
| Methylated C's in CpG context |
18386710 |
| Methylated C's in CHG context |
628262 |
| Methylated C's in CHH context |
8260723 |
| Methylated C's in Unknown context |
2658 |
| Unmethylated C's in CpG context |
3490698 |
| Unmethylated C's in CHG context |
22406312 |
| Unmethylated C's in CHH context |
72483174 |
| Unmethylated C's in Unknown context |
17691 |
| Percentage methylation (CpG context) |
84.0% |
| Percentage methylation (CHG context) |
2.7% |
| Percentage methylation (CHH context) |
10.2% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
4568710 |
original top strand |
| CTOT |
5564558 |
complementary to original top strand |
| CTOB |
5504500 |
complementary to original bottom strand |
| OB |
4543560 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
20181328 |
| Unique alignments |
11066467 |
| Duplicates removed |
9114861 |
| Duplicated alignments were found at 2605750 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
67599507 |
| Methylated C's in CpG context |
8137184 |
| Methylated C's in CHG context |
381105 |
| Methylated C's in CHH context |
5714033 |
| Unmethylated C's in CpG context |
1906366 |
| Unmethylated C's in CHG context |
11097562 |
| Unmethylated C's in CHH context |
40363257 |
| Percentage methylation (CpG context) |
81.0% |
| Percentage methylation (CHG context) |
3.3% |
| Percentage methylation (CHH context) |
12.4% |
|
|
M-Bias Plot