Alignment Stats
| Sequences analysed in total |
64352550 |
| Single-end alignments with a unique best hit |
15506461 |
| Sequences without alignments under any condition |
25878977 |
| Sequences that did not map uniquely |
22967112 |
| Genomic sequence context not extractable (edges of chromosomes) |
6509 |
|
|
Cytosine Methylation
| Total C's analysed |
97543916 |
| Methylated C's in CpG context |
14411420 |
| Methylated C's in CHG context |
496730 |
| Methylated C's in CHH context |
6618281 |
| Methylated C's in Unknown context |
2131 |
| Unmethylated C's in CpG context |
2660770 |
| Unmethylated C's in CHG context |
18387660 |
| Unmethylated C's in CHH context |
54969055 |
| Unmethylated C's in Unknown context |
11252 |
| Percentage methylation (CpG context) |
84.4% |
| Percentage methylation (CHG context) |
2.6% |
| Percentage methylation (CHH context) |
10.7% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
3492513 |
original top strand |
| CTOT |
4292667 |
complementary to original top strand |
| CTOB |
4246389 |
complementary to original bottom strand |
| OB |
3468383 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
15499952 |
| Unique alignments |
8610530 |
| Duplicates removed |
6889422 |
| Duplicated alignments were found at 2035597 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
53034062 |
| Methylated C's in CpG context |
6585746 |
| Methylated C's in CHG context |
300756 |
| Methylated C's in CHH context |
4550494 |
| Unmethylated C's in CpG context |
1448579 |
| Unmethylated C's in CHG context |
9225241 |
| Unmethylated C's in CHH context |
30923246 |
| Percentage methylation (CpG context) |
82.0% |
| Percentage methylation (CHG context) |
3.2% |
| Percentage methylation (CHH context) |
12.8% |
|
|
M-Bias Plot