Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_13_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 64352550
Single-end alignments with a unique best hit 15506461
Sequences without alignments under any condition 25878977
Sequences that did not map uniquely 22967112
Genomic sequence context not extractable (edges of chromosomes) 6509

Cytosine Methylation

Total C's analysed 97543916
Methylated C's in CpG context 14411420
Methylated C's in CHG context 496730
Methylated C's in CHH context 6618281
Methylated C's in Unknown context 2131
Unmethylated C's in CpG context 2660770
Unmethylated C's in CHG context 18387660
Unmethylated C's in CHH context 54969055
Unmethylated C's in Unknown context 11252
Percentage methylation (CpG context) 84.4%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 10.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3492513 original top strand
CTOT 4292667 complementary to original top strand
CTOB 4246389 complementary to original bottom strand
OB 3468383 original bottom strand

Deduplication

Alignments analysed 15499952
Unique alignments 8610530
Duplicates removed 6889422
Duplicated alignments were found at 2035597 different positions

Cytosine Methylation after Extraction

Total C's analysed 53034062
Methylated C's in CpG context 6585746
Methylated C's in CHG context 300756
Methylated C's in CHH context 4550494
Unmethylated C's in CpG context 1448579
Unmethylated C's in CHG context 9225241
Unmethylated C's in CHH context 30923246
Percentage methylation (CpG context) 82.0%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 12.8%

M-Bias Plot