Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_12_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 59459431
Single-end alignments with a unique best hit 15829417
Sequences without alignments under any condition 24225592
Sequences that did not map uniquely 19404422
Genomic sequence context not extractable (edges of chromosomes) 5509

Cytosine Methylation

Total C's analysed 95132801
Methylated C's in CpG context 10325372
Methylated C's in CHG context 419699
Methylated C's in CHH context 7034932
Methylated C's in Unknown context 1417
Unmethylated C's in CpG context 2774200
Unmethylated C's in CHG context 17728228
Unmethylated C's in CHH context 56850370
Unmethylated C's in Unknown context 10558
Percentage methylation (CpG context) 78.8%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 11.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3576347 original top strand
CTOT 4366365 complementary to original top strand
CTOB 4327570 complementary to original bottom strand
OB 3553626 original bottom strand

Deduplication

Alignments analysed 15823908
Unique alignments 10546952
Duplicates removed 5276956
Duplicated alignments were found at 2060083 different positions

Cytosine Methylation after Extraction

Total C's analysed 62811407
Methylated C's in CpG context 6103858
Methylated C's in CHG context 297572
Methylated C's in CHH context 5317105
Unmethylated C's in CpG context 1852012
Unmethylated C's in CHG context 11095947
Unmethylated C's in CHH context 38144913
Percentage methylation (CpG context) 76.7%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 12.2%

M-Bias Plot