Alignment Stats
| Sequences analysed in total |
59459431 |
| Single-end alignments with a unique best hit |
15829417 |
| Sequences without alignments under any condition |
24225592 |
| Sequences that did not map uniquely |
19404422 |
| Genomic sequence context not extractable (edges of chromosomes) |
5509 |
|
|
Cytosine Methylation
| Total C's analysed |
95132801 |
| Methylated C's in CpG context |
10325372 |
| Methylated C's in CHG context |
419699 |
| Methylated C's in CHH context |
7034932 |
| Methylated C's in Unknown context |
1417 |
| Unmethylated C's in CpG context |
2774200 |
| Unmethylated C's in CHG context |
17728228 |
| Unmethylated C's in CHH context |
56850370 |
| Unmethylated C's in Unknown context |
10558 |
| Percentage methylation (CpG context) |
78.8% |
| Percentage methylation (CHG context) |
2.3% |
| Percentage methylation (CHH context) |
11.0% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
3576347 |
original top strand |
| CTOT |
4366365 |
complementary to original top strand |
| CTOB |
4327570 |
complementary to original bottom strand |
| OB |
3553626 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
15823908 |
| Unique alignments |
10546952 |
| Duplicates removed |
5276956 |
| Duplicated alignments were found at 2060083 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
62811407 |
| Methylated C's in CpG context |
6103858 |
| Methylated C's in CHG context |
297572 |
| Methylated C's in CHH context |
5317105 |
| Unmethylated C's in CpG context |
1852012 |
| Unmethylated C's in CHG context |
11095947 |
| Unmethylated C's in CHH context |
38144913 |
| Percentage methylation (CpG context) |
76.7% |
| Percentage methylation (CHG context) |
2.6% |
| Percentage methylation (CHH context) |
12.2% |
|
|
M-Bias Plot