Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_11_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 54458572
Single-end alignments with a unique best hit 12385705
Sequences without alignments under any condition 22436878
Sequences that did not map uniquely 19635989
Genomic sequence context not extractable (edges of chromosomes) 6331

Cytosine Methylation

Total C's analysed 79508789
Methylated C's in CpG context 13425013
Methylated C's in CHG context 435393
Methylated C's in CHH context 5251496
Methylated C's in Unknown context 2688
Unmethylated C's in CpG context 2392973
Unmethylated C's in CHG context 14791894
Unmethylated C's in CHH context 43212020
Unmethylated C's in Unknown context 10045
Percentage methylation (CpG context) 84.9%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 10.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2798021 original top strand
CTOT 3421680 complementary to original top strand
CTOB 3375354 complementary to original bottom strand
OB 2784319 original bottom strand

Deduplication

Alignments analysed 12379374
Unique alignments 6289458
Duplicates removed 6089916
Duplicated alignments were found at 1477802 different positions

Cytosine Methylation after Extraction

Total C's analysed 39141996
Methylated C's in CpG context 5137792
Methylated C's in CHG context 240733
Methylated C's in CHH context 3470623
Unmethylated C's in CpG context 1278088
Unmethylated C's in CHG context 6581691
Unmethylated C's in CHH context 22433069
Percentage methylation (CpG context) 80.1%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 13.4%

M-Bias Plot