Alignment Stats
| Sequences analysed in total |
54458572 |
| Single-end alignments with a unique best hit |
12385705 |
| Sequences without alignments under any condition |
22436878 |
| Sequences that did not map uniquely |
19635989 |
| Genomic sequence context not extractable (edges of chromosomes) |
6331 |
|
|
Cytosine Methylation
| Total C's analysed |
79508789 |
| Methylated C's in CpG context |
13425013 |
| Methylated C's in CHG context |
435393 |
| Methylated C's in CHH context |
5251496 |
| Methylated C's in Unknown context |
2688 |
| Unmethylated C's in CpG context |
2392973 |
| Unmethylated C's in CHG context |
14791894 |
| Unmethylated C's in CHH context |
43212020 |
| Unmethylated C's in Unknown context |
10045 |
| Percentage methylation (CpG context) |
84.9% |
| Percentage methylation (CHG context) |
2.9% |
| Percentage methylation (CHH context) |
10.8% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
2798021 |
original top strand |
| CTOT |
3421680 |
complementary to original top strand |
| CTOB |
3375354 |
complementary to original bottom strand |
| OB |
2784319 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
12379374 |
| Unique alignments |
6289458 |
| Duplicates removed |
6089916 |
| Duplicated alignments were found at 1477802 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
39141996 |
| Methylated C's in CpG context |
5137792 |
| Methylated C's in CHG context |
240733 |
| Methylated C's in CHH context |
3470623 |
| Unmethylated C's in CpG context |
1278088 |
| Unmethylated C's in CHG context |
6581691 |
| Unmethylated C's in CHH context |
22433069 |
| Percentage methylation (CpG context) |
80.1% |
| Percentage methylation (CHG context) |
3.5% |
| Percentage methylation (CHH context) |
13.4% |
|
|
M-Bias Plot