Alignment Stats
| Sequences analysed in total |
78221457 |
| Single-end alignments with a unique best hit |
19946378 |
| Sequences without alignments under any condition |
31443747 |
| Sequences that did not map uniquely |
26831332 |
| Genomic sequence context not extractable (edges of chromosomes) |
8289 |
|
|
Cytosine Methylation
| Total C's analysed |
125999965 |
| Methylated C's in CpG context |
18462033 |
| Methylated C's in CHG context |
666868 |
| Methylated C's in CHH context |
8957808 |
| Methylated C's in Unknown context |
2711 |
| Unmethylated C's in CpG context |
3593439 |
| Unmethylated C's in CHG context |
25112918 |
| Unmethylated C's in CHH context |
69206899 |
| Unmethylated C's in Unknown context |
16306 |
| Percentage methylation (CpG context) |
83.7% |
| Percentage methylation (CHG context) |
2.6% |
| Percentage methylation (CHH context) |
11.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
5060955 |
original top strand |
| CTOT |
4959861 |
complementary to original top strand |
| CTOB |
4892743 |
complementary to original bottom strand |
| OB |
5024530 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
19938089 |
| Unique alignments |
6880960 |
| Duplicates removed |
13057129 |
| Duplicated alignments were found at 2958412 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
42318600 |
| Methylated C's in CpG context |
4775522 |
| Methylated C's in CHG context |
256958 |
| Methylated C's in CHH context |
4219604 |
| Unmethylated C's in CpG context |
1225901 |
| Unmethylated C's in CHG context |
7305355 |
| Unmethylated C's in CHH context |
24535260 |
| Percentage methylation (CpG context) |
79.6% |
| Percentage methylation (CHG context) |
3.4% |
| Percentage methylation (CHH context) |
14.7% |
|
|
M-Bias Plot