Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_10_s456_trimmed.fq

Data processed at 18:35 on 2022-06-19


Alignment Stats

Sequences analysed in total 78221457
Single-end alignments with a unique best hit 19946378
Sequences without alignments under any condition 31443747
Sequences that did not map uniquely 26831332
Genomic sequence context not extractable (edges of chromosomes) 8289

Cytosine Methylation

Total C's analysed 125999965
Methylated C's in CpG context 18462033
Methylated C's in CHG context 666868
Methylated C's in CHH context 8957808
Methylated C's in Unknown context 2711
Unmethylated C's in CpG context 3593439
Unmethylated C's in CHG context 25112918
Unmethylated C's in CHH context 69206899
Unmethylated C's in Unknown context 16306
Percentage methylation (CpG context) 83.7%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 11.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5060955 original top strand
CTOT 4959861 complementary to original top strand
CTOB 4892743 complementary to original bottom strand
OB 5024530 original bottom strand

Deduplication

Alignments analysed 19938089
Unique alignments 6880960
Duplicates removed 13057129
Duplicated alignments were found at 2958412 different positions

Cytosine Methylation after Extraction

Total C's analysed 42318600
Methylated C's in CpG context 4775522
Methylated C's in CHG context 256958
Methylated C's in CHH context 4219604
Unmethylated C's in CpG context 1225901
Unmethylated C's in CHG context 7305355
Unmethylated C's in CHH context 24535260
Percentage methylation (CpG context) 79.6%
Percentage methylation (CHG context) 3.4%
Percentage methylation (CHH context) 14.7%

M-Bias Plot