Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_all_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 605809374
Single-end alignments with a unique best hit 27806680
Sequences without alignments under any condition 550394238
Sequences that did not map uniquely 27608456
Genomic sequence context not extractable (edges of chromosomes) 671

Cytosine Methylation

Total C's analysed 172354157
Methylated C's in CpG context 27953676
Methylated C's in CHG context 967507
Methylated C's in CHH context 8116739
Methylated C's in Unknown context 972
Unmethylated C's in CpG context 7703035
Unmethylated C's in CHG context 30588122
Unmethylated C's in CHH context 97025078
Unmethylated C's in Unknown context 3699
Percentage methylation (CpG context) 78.4%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 7.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5864343 original top strand
CTOT 7523167 complementary to original top strand
CTOB 8088354 complementary to original bottom strand
OB 6330145 original bottom strand

Deduplication

Alignments analysed 27806009
Unique alignments 1129977
Duplicates removed 26676032
Duplicated alignments were found at 637756 different positions

Cytosine Methylation after Extraction

Total C's analysed 6688214
Methylated C's in CpG context 537926
Methylated C's in CHG context 50095
Methylated C's in CHH context 784788
Unmethylated C's in CpG context 276545
Unmethylated C's in CHG context 833957
Unmethylated C's in CHH context 4204903
Percentage methylation (CpG context) 66.0%
Percentage methylation (CHG context) 5.7%
Percentage methylation (CHH context) 15.7%

M-Bias Plot