Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_9_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 77350922
Single-end alignments with a unique best hit 3614877
Sequences without alignments under any condition 69851458
Sequences that did not map uniquely 3884587
Genomic sequence context not extractable (edges of chromosomes) 89

Cytosine Methylation

Total C's analysed 22085171
Methylated C's in CpG context 2591728
Methylated C's in CHG context 102822
Methylated C's in CHH context 1045017
Methylated C's in Unknown context 74
Unmethylated C's in CpG context 1010302
Unmethylated C's in CHG context 3818600
Unmethylated C's in CHH context 13516702
Unmethylated C's in Unknown context 741
Percentage methylation (CpG context) 72.0%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 7.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 743807 original top strand
CTOT 999563 complementary to original top strand
CTOB 1061481 complementary to original bottom strand
OB 809937 original bottom strand

Deduplication

Alignments analysed 3614788
Unique alignments 481549
Duplicates removed 3133239
Duplicated alignments were found at 235196 different positions

Cytosine Methylation after Extraction

Total C's analysed 2945477
Methylated C's in CpG context 251709
Methylated C's in CHG context 16074
Methylated C's in CHH context 272980
Unmethylated C's in CpG context 128793
Unmethylated C's in CHG context 389241
Unmethylated C's in CHH context 1886680
Percentage methylation (CpG context) 66.2%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 12.6%

M-Bias Plot