Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_8_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 38697189
Single-end alignments with a unique best hit 1779165
Sequences without alignments under any condition 35045244
Sequences that did not map uniquely 1872780
Genomic sequence context not extractable (edges of chromosomes) 58

Cytosine Methylation

Total C's analysed 10983738
Methylated C's in CpG context 1642871
Methylated C's in CHG context 61069
Methylated C's in CHH context 546466
Methylated C's in Unknown context 65
Unmethylated C's in CpG context 439184
Unmethylated C's in CHG context 1942783
Unmethylated C's in CHH context 6351365
Unmethylated C's in Unknown context 292
Percentage methylation (CpG context) 78.9%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 7.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 357923 original top strand
CTOT 504571 complementary to original top strand
CTOB 529862 complementary to original bottom strand
OB 386751 original bottom strand

Deduplication

Alignments analysed 1779107
Unique alignments 314494
Duplicates removed 1464613
Duplicated alignments were found at 142349 different positions

Cytosine Methylation after Extraction

Total C's analysed 1963151
Methylated C's in CpG context 213026
Methylated C's in CHG context 11872
Methylated C's in CHH context 183281
Unmethylated C's in CpG context 74232
Unmethylated C's in CHG context 269542
Unmethylated C's in CHH context 1211198
Percentage methylation (CpG context) 74.2%
Percentage methylation (CHG context) 4.2%
Percentage methylation (CHH context) 13.1%

M-Bias Plot