Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_7_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 49730113
Single-end alignments with a unique best hit 2247688
Sequences without alignments under any condition 45094621
Sequences that did not map uniquely 2387804
Genomic sequence context not extractable (edges of chromosomes) 64

Cytosine Methylation

Total C's analysed 13889878
Methylated C's in CpG context 2000903
Methylated C's in CHG context 75085
Methylated C's in CHH context 662881
Methylated C's in Unknown context 58
Unmethylated C's in CpG context 635805
Unmethylated C's in CHG context 2459263
Unmethylated C's in CHH context 8055941
Unmethylated C's in Unknown context 331
Percentage methylation (CpG context) 75.9%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 7.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 467398 original top strand
CTOT 615887 complementary to original top strand
CTOB 656717 complementary to original bottom strand
OB 507622 original bottom strand

Deduplication

Alignments analysed 2247624
Unique alignments 368773
Duplicates removed 1878851
Duplicated alignments were found at 171559 different positions

Cytosine Methylation after Extraction

Total C's analysed 2285525
Methylated C's in CpG context 231457
Methylated C's in CHG context 14129
Methylated C's in CHH context 213306
Unmethylated C's in CpG context 97876
Unmethylated C's in CHG context 310464
Unmethylated C's in CHH context 1418293
Percentage methylation (CpG context) 70.3%
Percentage methylation (CHG context) 4.4%
Percentage methylation (CHH context) 13.1%

M-Bias Plot