Alignment Stats
| Sequences analysed in total |
49730113 |
| Single-end alignments with a unique best hit |
2247688 |
| Sequences without alignments under any condition |
45094621 |
| Sequences that did not map uniquely |
2387804 |
| Genomic sequence context not extractable (edges of chromosomes) |
64 |
|
|
Cytosine Methylation
| Total C's analysed |
13889878 |
| Methylated C's in CpG context |
2000903 |
| Methylated C's in CHG context |
75085 |
| Methylated C's in CHH context |
662881 |
| Methylated C's in Unknown context |
58 |
| Unmethylated C's in CpG context |
635805 |
| Unmethylated C's in CHG context |
2459263 |
| Unmethylated C's in CHH context |
8055941 |
| Unmethylated C's in Unknown context |
331 |
| Percentage methylation (CpG context) |
75.9% |
| Percentage methylation (CHG context) |
3.0% |
| Percentage methylation (CHH context) |
7.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
467398 |
original top strand |
| CTOT |
615887 |
complementary to original top strand |
| CTOB |
656717 |
complementary to original bottom strand |
| OB |
507622 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
2247624 |
| Unique alignments |
368773 |
| Duplicates removed |
1878851 |
| Duplicated alignments were found at 171559 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
2285525 |
| Methylated C's in CpG context |
231457 |
| Methylated C's in CHG context |
14129 |
| Methylated C's in CHH context |
213306 |
| Unmethylated C's in CpG context |
97876 |
| Unmethylated C's in CHG context |
310464 |
| Unmethylated C's in CHH context |
1418293 |
| Percentage methylation (CpG context) |
70.3% |
| Percentage methylation (CHG context) |
4.4% |
| Percentage methylation (CHH context) |
13.1% |
|
|
M-Bias Plot